Jiarti Kusbandiyah, Mahmudah, Su Peng Loh, Yuniar Angelia Puspadewi, Ari Damayanti Wahyuningrum, Dinda Oktia Maghfiroh
Introduction: Anemia is a malnutrition condition with high prevalence in the world, especially in developing countries. Adequate intake of macro and micro nutrients is absolutely necessary, in the form of food consumption or extracts. This study aims to analyze the effect of a combination of red spinach and chrysanthemum flower ethanol extracts on hemoglobin and hepcidin levels. Materials and methods: This research design uses a laboratory experimental design with a posttest -only control group design in vivo. This study used 24 teenage female Wistar rats (ratus norvegicus) divided into 6 groups. The treatment was administration of EEBM+EEBK in 4 doses, EEBM 300 mg + EEBK 300 mg, EEBM 600 mg + EEBK 300 mg, EEBM 900 mg + EEBK 300 mg and EEBM 1200 mg + EEBK 300 mg. The evaluation of hemoglobin and hepcidin levels was carried out for nine days. Research data is displayed in the form of Mean ± SD. The research results were analyzed using one-way ANOVA with Tukey's Post-Hoc test. Results: The results of the One-Way ANOVA statistical test showed that there were significant differences for Hb (p=0.000) and hepcidin (p=0.007). The most significant dose capable of increasing Hb levels and reducing hepcidin levels was combination of EEBM 600 mg + EEBK 300 mg. Conclusion: The combination of red spinach and chrysanthemum flower ethanol extracts shows promise as an alternative for managing hemolytic anemia in adolescents. However, further research directly on human subjects is warranted to validate these findings. © 2025 Universiti Putra Malaysia Press. All rights reserved.
Doctoral Study Program, Faculty of Public Health, Airlangga University, Surabaya, 60115, Indonesia; Midwifery Study Program, Widyagama Husada School of Health, Malang, 65142, Indonesia; Nursing Study Program, Widyagama Husada School of Health, Malang, 65142, Indonesia; Department of Nutrition, Faculty of Medicine and Health Science, Universiti Putra Malaysia, Selangor, Serdang, 43400, Malaysia
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